I found a solition ,

asp.net configration;

public class Program
    {
        public static void Main(string[] args)
        {
            BuildWebHost(args).Run();
        }

        public static IWebHost BuildWebHost(string[] args) =>
            WebHost.CreateDefaultBuilder(args)
                .UseStartup<Startup>()
                 .UseUrls("http://localhost:5001/")    // We are here to work at a different port
                .Build();
    }

nginx configration

server_name  yourdomain.com ;
 location / {

                 proxy_pass http://localhost:5001; // Listening 5001 Port
                 proxy_http_version 1.1;
                 proxy_set_header Upgrade $http_upgrade;
                 proxy_set_header Connection keep-alive;
                 proxy_set_header Host $http_host;
                 proxy_cache_bypass $http_upgrade;

                # First attempt to serve request as file, then
                # as directory, then fall back to displaying a 404.
                # try_files $uri $uri/ =404;
        }